TUBB anticorps

N° du produit ABIN238519

Cet anticorps Souris Monoclonal détecte spécifiquement TUBB dans WB, IF, IHC (fro) et EIA. Il présente une réactivité envers Humain, Souris, Porc et Plantes.

Aperçu rapide pour TUBB anticorps (ABIN238519)

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Humain, Souris, Porc, Plantes

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: TU-13

Détail du produit anti-TUBB anticorps

Specificité: The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.

Attributs du produit: Synonyms: Tubulin beta chain, Tubulin beta-5 chain

Purification: Precipitation Methods and Size-Exclusion Chromatography.

Immunogène: beta-tubulin from porcine brain.

Isotype: IgM

Information d'application

Indications d'application: Western Blotting (Reducing Conditions): 1 μg/mL, Sonication of MCF7 cells isrecommended. Positive Control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch

Date de péremption: 12 months

Détails sur TUBB

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: TUBB / TUBB5

Sujet: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.Synonyms: Tubulin beta chain, Tubulin beta-5 chain

ID gène: 203068

UniProt: P07437

Pathways: Dynamique des Microtubules, M Phase